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plasmid pdual-hff1  (BioResource International Inc)

 
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    Structured Review

    BioResource International Inc plasmid pdual-hff1
    Plasmid Pdual Hff1, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+pdual-hff1/plasmid+pdual+hff1/pmc11276115-60-1-4
    Average 90 stars, based on 1 article reviews
    plasmid pdual-hff1 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Revealing Differential RNA Editing Specificity of Human ADAR1 and ADAR2 in Schizosaccharomyces pombe
    Article Snippet: The plasmid pDUAL-HFF1 (RIKEN BioResource Research Center, Tsukuba, Japan) was digested with the Nhe I/ Bgl II restriction enzymes (New England Biolabs, Ipswich, MA, USA), before it was assembled with the amplified hADAR1 or hADAR2 PCR fragments, respectively, producing plasmids pDUAL-HFF1-hADAR1 and pDUAL-HFF1-hADAR2.

    Article Title: DeepEdit: single-molecule detection and phasing of A-to-I RNA editing events using nanopore direct RNA sequencing
    Article Snippet: The plasmid pDUAL-HFF1-hADAR2 was constructed by inserting the hADAR2 coding sequences into the NheI/BglII site of plasmid pDUAL-HFF1 (RIKEN BioResource Center, Ibaraki, Japan).

    Article Title: Interfering with retrotransposition by two types of CRISPR effectors: Cas12a and Cas13a
    Article Snippet: The plasmid pDUAL-HFF1 (RIKEN BioResource Center, Ibaraki, Japan) was digested with the Nde I/ Nco I restriction enzymes, before it was assembled with the above amplified FnCpf1 or LbCpf1 PCR fragments, respectively, using ClonExpress® II One Step Cloning Kit (Vazyme), producing plasmids pDUAL-HFF1-FnCpf1 and pDUAL-HFF1-LbCpf1 (Supplementary Fig. and Table ).

    Article Title: DeepEdit: single-molecule detection and phasing of A-to-I RNA editing events using nanopore direct RNA sequencing.
    Article Snippet: The plasmid pDUALHFF1-hADAR2 was constructed by inserting the hADAR2 coding sequences into the NheI/BglII site of plasmid pDUAL-HFF1 (RIKEN BioResource Center, Ibaraki, Japan).

    Amplification:

    Article Title: Revealing Differential RNA Editing Specificity of Human ADAR1 and ADAR2 in Schizosaccharomyces pombe
    Article Snippet: The plasmid pDUAL-HFF1 (RIKEN BioResource Research Center, Tsukuba, Japan) was digested with the Nhe I/ Bgl II restriction enzymes (New England Biolabs, Ipswich, MA, USA), before it was assembled with the amplified hADAR1 or hADAR2 PCR fragments, respectively, producing plasmids pDUAL-HFF1-hADAR1 and pDUAL-HFF1-hADAR2.

    Article Title: DeepEdit: single-molecule detection and phasing of A-to-I RNA editing events using nanopore direct RNA sequencing
    Article Snippet: The plasmid pDUAL-HFF1-hADAR2 was constructed by inserting the hADAR2 coding sequences into the NheI/BglII site of plasmid pDUAL-HFF1 (RIKEN BioResource Center, Ibaraki, Japan).

    Article Title: Interfering with retrotransposition by two types of CRISPR effectors: Cas12a and Cas13a
    Article Snippet: The plasmid pDUAL-HFF1 (RIKEN BioResource Center, Ibaraki, Japan) was digested with the Nde I/ Nco I restriction enzymes, before it was assembled with the above amplified FnCpf1 or LbCpf1 PCR fragments, respectively, using ClonExpress® II One Step Cloning Kit (Vazyme), producing plasmids pDUAL-HFF1-FnCpf1 and pDUAL-HFF1-LbCpf1 (Supplementary Fig. and Table ).

    Article Title: DeepEdit: single-molecule detection and phasing of A-to-I RNA editing events using nanopore direct RNA sequencing.
    Article Snippet: The plasmid pDUALHFF1-hADAR2 was constructed by inserting the hADAR2 coding sequences into the NheI/BglII site of plasmid pDUAL-HFF1 (RIKEN BioResource Center, Ibaraki, Japan).

    Polymerase Chain Reaction:

    Article Title: Revealing Differential RNA Editing Specificity of Human ADAR1 and ADAR2 in Schizosaccharomyces pombe
    Article Snippet: The plasmid pDUAL-HFF1 (RIKEN BioResource Research Center, Tsukuba, Japan) was digested with the Nhe I/ Bgl II restriction enzymes (New England Biolabs, Ipswich, MA, USA), before it was assembled with the amplified hADAR1 or hADAR2 PCR fragments, respectively, producing plasmids pDUAL-HFF1-hADAR1 and pDUAL-HFF1-hADAR2.

    Article Title: DeepEdit: single-molecule detection and phasing of A-to-I RNA editing events using nanopore direct RNA sequencing
    Article Snippet: The plasmid pDUAL-HFF1-hADAR2 was constructed by inserting the hADAR2 coding sequences into the NheI/BglII site of plasmid pDUAL-HFF1 (RIKEN BioResource Center, Ibaraki, Japan).

    Article Title: Interfering with retrotransposition by two types of CRISPR effectors: Cas12a and Cas13a
    Article Snippet: The plasmid pDUAL-HFF1 (RIKEN BioResource Center, Ibaraki, Japan) was digested with the Nde I/ Nco I restriction enzymes, before it was assembled with the above amplified FnCpf1 or LbCpf1 PCR fragments, respectively, using ClonExpress® II One Step Cloning Kit (Vazyme), producing plasmids pDUAL-HFF1-FnCpf1 and pDUAL-HFF1-LbCpf1 (Supplementary Fig. and Table ).

    Article Title: DeepEdit: single-molecule detection and phasing of A-to-I RNA editing events using nanopore direct RNA sequencing.
    Article Snippet: The plasmid pDUALHFF1-hADAR2 was constructed by inserting the hADAR2 coding sequences into the NheI/BglII site of plasmid pDUAL-HFF1 (RIKEN BioResource Center, Ibaraki, Japan).

    Construct:

    Article Title: Revealing Differential RNA Editing Specificity of Human ADAR1 and ADAR2 in Schizosaccharomyces pombe
    Article Snippet: The plasmid pDUAL-HFF1 (RIKEN BioResource Research Center, Tsukuba, Japan) was digested with the Nhe I/ Bgl II restriction enzymes (New England Biolabs, Ipswich, MA, USA), before it was assembled with the amplified hADAR1 or hADAR2 PCR fragments, respectively, producing plasmids pDUAL-HFF1-hADAR1 and pDUAL-HFF1-hADAR2.

    Article Title: DeepEdit: single-molecule detection and phasing of A-to-I RNA editing events using nanopore direct RNA sequencing
    Article Snippet: The plasmid pDUAL-HFF1-hADAR2 was constructed by inserting the hADAR2 coding sequences into the NheI/BglII site of plasmid pDUAL-HFF1 (RIKEN BioResource Center, Ibaraki, Japan).

    Article Title: Interfering with retrotransposition by two types of CRISPR effectors: Cas12a and Cas13a
    Article Snippet: The plasmid pDUAL-HFF1 (RIKEN BioResource Center, Ibaraki, Japan) was digested with the Nde I/ Nco I restriction enzymes, before it was assembled with the above amplified FnCpf1 or LbCpf1 PCR fragments, respectively, using ClonExpress® II One Step Cloning Kit (Vazyme), producing plasmids pDUAL-HFF1-FnCpf1 and pDUAL-HFF1-LbCpf1 (Supplementary Fig. and Table ).

    Article Title: DeepEdit: single-molecule detection and phasing of A-to-I RNA editing events using nanopore direct RNA sequencing.
    Article Snippet: The plasmid pDUALHFF1-hADAR2 was constructed by inserting the hADAR2 coding sequences into the NheI/BglII site of plasmid pDUAL-HFF1 (RIKEN BioResource Center, Ibaraki, Japan).

    Cloning:

    Article Title: Revealing Differential RNA Editing Specificity of Human ADAR1 and ADAR2 in Schizosaccharomyces pombe
    Article Snippet: The plasmid pDUAL-HFF1 (RIKEN BioResource Research Center, Tsukuba, Japan) was digested with the Nhe I/ Bgl II restriction enzymes (New England Biolabs, Ipswich, MA, USA), before it was assembled with the amplified hADAR1 or hADAR2 PCR fragments, respectively, producing plasmids pDUAL-HFF1-hADAR1 and pDUAL-HFF1-hADAR2.

    Article Title: DeepEdit: single-molecule detection and phasing of A-to-I RNA editing events using nanopore direct RNA sequencing
    Article Snippet: The plasmid pDUAL-HFF1-hADAR2 was constructed by inserting the hADAR2 coding sequences into the NheI/BglII site of plasmid pDUAL-HFF1 (RIKEN BioResource Center, Ibaraki, Japan).

    Article Title: Interfering with retrotransposition by two types of CRISPR effectors: Cas12a and Cas13a
    Article Snippet: The plasmid pDUAL-HFF1 (RIKEN BioResource Center, Ibaraki, Japan) was digested with the Nde I/ Nco I restriction enzymes, before it was assembled with the above amplified FnCpf1 or LbCpf1 PCR fragments, respectively, using ClonExpress® II One Step Cloning Kit (Vazyme), producing plasmids pDUAL-HFF1-FnCpf1 and pDUAL-HFF1-LbCpf1 (Supplementary Fig. and Table ).

    Article Title: DeepEdit: single-molecule detection and phasing of A-to-I RNA editing events using nanopore direct RNA sequencing.
    Article Snippet: The plasmid pDUALHFF1-hADAR2 was constructed by inserting the hADAR2 coding sequences into the NheI/BglII site of plasmid pDUAL-HFF1 (RIKEN BioResource Center, Ibaraki, Japan).



    Similar Products

    plasmid pdual-hff1  (BioResource International Inc)
    90
    BioResource International Inc plasmid pdual-hff1
    Plasmid Pdual Hff1, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+pdual-hff1/plasmid+pdual+hff1/pmc11276115-60-1-4
    Average 90 stars, based on 1 article reviews
    plasmid pdual-hff1 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    plasmids pdual hff1 fncpf1  (Thermo Fisher)
    86
    Thermo Fisher plasmids pdual hff1 fncpf1
    a Schematic representation of the S. pombe chromosomal locus with integrated Cas12a constructs. b Growth in liquid culture media (EMM + uracil) of S. pombe strains expressing <t>FnCpf1</t> or LbCpf1, compared to the control strain with blank insertion at the same locus. They had an average double time of 4.1, 4.6 and 4.5 h, respectively. Data are means ± SEM ( n = 4 independent experiments). c Schematic representation of MEL1 gene editing. crRNAs were designed to target five sites (NT1, NT2, T3, T4, and T5) within MEL1 gene. The DNA cut by Cas12a is repaired with the repair fragment by HR. The arrows denote the primers, MEL1-P5 and MEL1-P3, and their PCR products for diagnostic experiments. d The editing efficiency on MEL1 gene with five different crRNAs, estimated by formation of white- and blue-colored colonies, assayed on agar plates containing the X-α-Gal. e Efficiency of genome editing on MEL1 gene with five different crRNAs. Values are mean ± SEM, n = 3. f Diagnostic PCR products of mel1 mutants (white colonies) generated from FnCpf1 editing, showing 998-bp in size. They are compared with the wild-type (WT) product, 3036-bp in size. Primer sets MEL1-P5 and MEL1-P3 ( c ) were used for PCR.
    Plasmids Pdual Hff1 Fncpf1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+pdual-hff1/pmc07235257-189-10-29
    Average 86 stars, based on 1 article reviews
    plasmids pdual hff1 fncpf1 - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    a Schematic representation of the S. pombe chromosomal locus with integrated Cas12a constructs. b Growth in liquid culture media (EMM + uracil) of S. pombe strains expressing FnCpf1 or LbCpf1, compared to the control strain with blank insertion at the same locus. They had an average double time of 4.1, 4.6 and 4.5 h, respectively. Data are means ± SEM ( n = 4 independent experiments). c Schematic representation of MEL1 gene editing. crRNAs were designed to target five sites (NT1, NT2, T3, T4, and T5) within MEL1 gene. The DNA cut by Cas12a is repaired with the repair fragment by HR. The arrows denote the primers, MEL1-P5 and MEL1-P3, and their PCR products for diagnostic experiments. d The editing efficiency on MEL1 gene with five different crRNAs, estimated by formation of white- and blue-colored colonies, assayed on agar plates containing the X-α-Gal. e Efficiency of genome editing on MEL1 gene with five different crRNAs. Values are mean ± SEM, n = 3. f Diagnostic PCR products of mel1 mutants (white colonies) generated from FnCpf1 editing, showing 998-bp in size. They are compared with the wild-type (WT) product, 3036-bp in size. Primer sets MEL1-P5 and MEL1-P3 ( c ) were used for PCR.

    Journal: Cell Discovery

    Article Title: Interfering with retrotransposition by two types of CRISPR effectors: Cas12a and Cas13a

    doi: 10.1038/s41421-020-0164-0

    Figure Lengend Snippet: a Schematic representation of the S. pombe chromosomal locus with integrated Cas12a constructs. b Growth in liquid culture media (EMM + uracil) of S. pombe strains expressing FnCpf1 or LbCpf1, compared to the control strain with blank insertion at the same locus. They had an average double time of 4.1, 4.6 and 4.5 h, respectively. Data are means ± SEM ( n = 4 independent experiments). c Schematic representation of MEL1 gene editing. crRNAs were designed to target five sites (NT1, NT2, T3, T4, and T5) within MEL1 gene. The DNA cut by Cas12a is repaired with the repair fragment by HR. The arrows denote the primers, MEL1-P5 and MEL1-P3, and their PCR products for diagnostic experiments. d The editing efficiency on MEL1 gene with five different crRNAs, estimated by formation of white- and blue-colored colonies, assayed on agar plates containing the X-α-Gal. e Efficiency of genome editing on MEL1 gene with five different crRNAs. Values are mean ± SEM, n = 3. f Diagnostic PCR products of mel1 mutants (white colonies) generated from FnCpf1 editing, showing 998-bp in size. They are compared with the wild-type (WT) product, 3036-bp in size. Primer sets MEL1-P5 and MEL1-P3 ( c ) were used for PCR.

    Article Snippet: For chromosomal integration of FnCpf1 and LbCpf1 expression constructs, the plasmids pDUAL-HFF1-FnCpf1 and pDUAL-HFF1-LbCpf1 were digested with the Not I restriction enzyme, and treated with FastAP Thermosensitive Alkaline Phosphatase (Thermo Scientific, Shanghai) before linearized DNA was recovered from agarose gel electrophoresis for yeast transformation.

    Techniques: Construct, Expressing, Diagnostic Assay, Generated